sql server - ASP.NET Application time out error -

Please help me, I have time & amp; This application was very good for the last 5 years, using SQL Server 2005 as a presence application database in ASP.X, for the last 5 years, it is getting data for the first time inquiries, but It is taking a long time for the second query and is finally showing the error from time to time

Recently updated my system admin to Windows, Donnet & amp; Sqlserver hotfixes

Rebuild your indexes and statistics.

See.

[Which version of SQL Server BTW?]